The Cell Wall Barrier of Corynebacterium glutamicum and Amino Acid Efflux.

摘要: Abstract Corynebacterium glutamicum is extremely well suited for the production of amino acids, and way in which biosynthesis pathways have to be engineered this purpose very understood. However, special significance cell envelope as a barrier process only just being recognized. In addition it determines cellular synthesis capacity. The wall Corynebacterianeae , also include Mycobacterium tuberculosis has complex structure first detailed findings on their are available. ubiquitous inner lipid bilayer, an outer layer contains mycolic acids probably organized bilayer. During export, acid pass these different layers wall. Molecular investigations now identified l -lysine exporter LysE -threonine ThrE localized cytoplasmic It was revealed that both carriers represent prototype previously unknown translocator families. This involves extended families whose members present bacteria archeae. exports -arginine. Its expression regulated by elevated concentration cell-internal acid, may, example, case presence peptides. Export thus represents new bacterial mechanism regulating balance. export -glutamic still enigmatic, although seems play major role efflux acid. Very surprisingly treatments, such detergents, but penicillin, always required order obtain high -glutamate. assumed ultimate target additions primarily layer. individual twenty might various quite ways. A challenge future work discover how takes place detail then apply further strain improvement.