Purification and structure analysis of mycolic acids in Corynebacterium glutamicum.
作者： Yang Yang , Feng Shi , Guanjun Tao , Xiaoyuan Wang
DOI: 10.1007/S12275-012-1459-0
关键词:
摘要: Corynebacterium glutamicum is widely used for producing amino acids. Mycolic acids, the major components in cell wall of C. might be closely related to secretion In this study, mycolic acids were extracted from 5 strains glutamicum, including ATCC 13032, 13869, 14067, L-isoleucine strain IWJ-1, and L-valine VWJ-1. Structures these analyzed using thin layer chromatography electrospray ionization mass spectrometry. More than twenty molecular species acid observed all strains. They differ length (20–40 carbons) saturation (0–3 double bonds) their constituent fatty The dominant every was different, but two hydrocarbon chains similar (14–18 carbons), meromycolate chain usually contained bonds. As growth temperature cells increased 30°C 34°C, proportion containing unsaturated shorter increased. These results provide new information on could useful modifying increase production
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