Single-Molecule Fluorescent Particle Tracking

摘要: One of the most fascinating processes in biology is directed movement organisms, subcellular compartments, and single proteins. Tracking cellular motion great interest to single-molecule biophysicists understand mechanism wide variety biological processes, from basic molecular machines protein--protein interactions. In last two decades, random diffusion proteins lipids has been tracked under fluorescence microscope how they associate with their targeted molecules. However, motility not limited small particles. Many fundamental occur by discrete physical movements upon each enzymatic cycle. For example, motor cytoskeleton can transport intracellular cargoes taking nanometer-sized steps along linear tracks within cell. Several high precision techniques have developed working principles kinetics motors a detailed manner. This chapter summarizes recent advances microscopy that allow tracking