Differentiating Large Cell Lymphoma from Indolent Small B-Cell Lymphoma in Fine Needle Aspirates Using p53, PCNA and Transformed Lymphocyte Count

摘要: OBJECTIVE: To determine the usefulness of proliferating cell nuclear antigen (PCNA), p53 protein expression and transformed lymphocyte count (TLC) as adjunctive tests to differentiate indolent small B-cell lymphoma from large in fine needle aspiration biopsies. STUDY DESIGN: Aspirates lymphoproliferative disorders April 1993 January 1997 were reviewed. The percentage TLCs was determined on Papanicolaou smear. intensity PCNA immunocytochemical staining evaluated block sections. These results compared correlated with final diagnoses based available morphology, flow cytometry clinical history. RESULTS: There 40 cases non-Hodgkin's 12 reactive lymph nodes. Adequate blocks 16 lymphomas, 7 grade 1-2 follicular center 6 mucosal associated lymphoid tissue 2 lymphocytic lymphomas mantle lymphomas. Average TLC highest (57% 24% p53), followed by grades 1 (14% 15% p53) lowest other (<10% <1% p53). (46%) (7%); however, best parameter for differentiating lymphoma. CONCLUSION: differentiated better than either or alone combination. Significant overlap between categories limits these stains entities.