Molecular Imaging with Bioluminescence and PET Reveals Viral Oncolysis Kinetics and Tumor Viability

摘要: Viral oncolysis, the destruction of cancer cells by replicating virus, is an experimental therapy that continues to be explored. The treatment paradigm for this involves successive waves lytic replication in cells. At current, monitoring viral titer at sites requires biopsy. However, repeat serial biopsies are not practically feasible temporal and tumor response patients. Molecular imaging provides a non-invasive method identify intracellular gene expression real time. We imaged oncolysis sequentially with bioluminescence positron emission tomography (PET), revealing kinetics both processes xenografts. demonstrate virus cycles can identified as reporter occur ~2 days after initial infection peak. These correspond virions released following cycle. cellular were Fluc Rluc reporters plus two 18F-labelled PET (FHBG, 9-(4-18F-fluoro-3-[hydroxymethyl] butyl) guanine) (FLT, 18F- 3′-deoxy-3-′fluorothymidine), respectively. Correlative immunohistochemistry on xenograft sections confirmed vivo results. Our findings show how used real-time measurements intratumoral levels. This noninvasive approach has potential utility