Modulations of hepatic gamma-glutamyltranspeptidase and N-hydroxy-N-2-fluorenylacetamide sulfotransferase activities following treatment of rats with a hepatocarcinogenic regimen: effect of partial hepatectomy.

摘要: The feasibility of using biochemical assays gamma-glutamyltranspeptidase (gamma-GTP) and N-hydroxy-N-2-fluorenylacetamide sulfotransferase (N-OH-2-FAA ST) activities to monitor the effects treatment male Sprague-Dawley rats with a two-stage hepatocarcinogenic regimen was investigated. One week after initiation diethylnitrosamine (200 mg/kg bw), were treated 10 oral doses within 2 weeks N-2-fluorenylacetamide (2-FAA) at 0.05 mmole/kg or vehicle (corn oil) 5 ml/kg body weight. After five 2-FAA corn oil, half in each group underwent partial (70%) hepatectomy (PH). Three days completion treatment, gamma-GTP activity increased approximately 8-fold livers both nonhepatectomized (-PH) hepatectomized (+PH) groups. 17 days, enzyme decreased control level -PH but 3.1-fold above +PH group. 31, 66, 87 only 1.4- 2.6-fold group, whereas that 15- 32-fold. N-OH-2-FAA ST activity, determined 3 by 60% effect PH became evident losses smaller (20%) than (45.5%). respective decreases 27, 29, 41% significant.(ABSTRACT TRUNCATED AT 250 WORDS)