The role of N-sulfation in the N-hydroxy-2-acetylaminofluorene-mediated outgrowth of diethylnitrosamine-initiated hepatocytes to γ-glutamyltranspeptidase-positive foci in male rat liver

摘要: The ability of N-hydroxy-2-acetylaminofluorene (N-OH-AAF) to promote the appearance gamma-glutamyltranspeptidase-positive (GGT+) foci initiated by diethylnitrosamine (DEN) was studied in a slightly modified Solt and Farber protocol. This protocol consisted following treatments: initiation with single dose DEN followed selection/promotion several non-necrogenic doses N-OH-AAF partial hepatectomy. Treatment resulted 25-fold increase liver volume occupied GGT+ cells as compared controls. role N-sulfation foci-selecting activity using sulfotransferase inhibitor pentachlorophenol (PCP). Inhibition pathway PCP during selection greater than 80% decrease cells, without effects on number generated this Also, reduced so called oval bile duct N-OH-AAF/partial hepatectomy treatment. It is concluded that responsible for N-OH-AAF-mediated outgrowth DEN-initiated hepatocytes preneoplastic foci.