Bladder Surface Glycosaminoglycans: An Epithelial Permeability Barrier

作者: L. Parsons , D. Boychuk , S. Jones , R. Hurst , H. Callahan

DOI: 10.1016/S0022-5347(17)39897-X

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摘要: Sulfated polysaccharide's ability to modulate the movement of small molecules was examined both in vivo and vitro. For test, rabbit bladder utilized C-14 labeled urea 45-Ca, or 3H2O placed into lumen control bladders, bladders pretreated with protamine sulfate (20 mg./cc) plus pentosanpoly-sulfate (PPS), 10 mg./cc. After 45 minutes, controls absorbed 21% urea, 16% calcium, 38% 3H2O; treated group 40% 23% 51% H2O; PPS only 22% 24% 18% 44% water. Differences between groups were statistically significant, p less than 0.01 for 45-Ca 3H2O. The mucosa contained a significantly higher concentration calcium after treatment which reversed by (p 0.01) while content went down = 0.03), reflecting loss hydrophilic effect GAG. mucosas had 250 cpm/mg. tissue Ca 64 water 262 cpm/mg., 498 190 H2O 139 cpm/mg.; 344 129 168 vitro studies, an Ussing chamber employed. Normal membranes chambers potential difference zeroed across membrane. There three groups, that irrigating solution, protamine, PPS. At end 40 there approximately 1.2% membrane, 3.5% membrane (a significant increase 0.001) 1.1% It would appear surface polysaccharide may play important role as permeability barrier modulating charged uncharged molecule its impair such can be inhibited this exogenous sulfated polysaccharide.

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