Systems and methods for barcoding nucleic acids

作者: David A Weitz , Allon Moshe Klein , Ilke Akartuna , Linas Mazutis , Marc W Kirschner

DOI:

关键词:

摘要: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are directed systems methods for labeling acids within microfluidic droplets. In one set of embodiments, the may include "barcodes" or unique sequences that can be used distinguish in a droplet from those another droplet, instance, even after pooled together. some cases, incorporated into individual droplets using particles attached contained (for released lysed cells). barcodes tens, hundreds, thousands acids, e.g., arising different cells other sources.

参考文章(139)
Michael Samuels, Darren Roy Link, Sandwich assays in droplets ,(2011)
Kevin S. Eng, Kanwar K. Singh, Feng Chen, Jeff L. Froula, Tao Zhang, Len A. Pennacchio, Long-Range Barcode Labeling-Sequencing ,(2012)
Maryam Shariati, David Liu, Joon Mo Yang, Sam Woo, Dennis A. Lehto, David M. Cox, System comprising dual-sided thermal cycler and emulsion pcr in pouch ,(2010)
David A. Weitz, John Heyman, Systems and methods of droplet-based selection ,(2009)
Fabio Fachin, Brian Wardle, Mehmet Toner, Grace Chen, Microscale and nanoscale structures for manipulating particles ,(2011)
Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Michael Schnall-Levin, Mirna Jarosz, Methods and systems for processing polynucleotides ,(2013)
David A Weitz, Jeremy Agresti, Michael P. Weiner, Adam R. Abate, Tony Hung, Systems and methods for nucleic acid sequencing ,(2008)
Peter Hong, Andrei Alexeev, Radoje Drmanac, Brock A. Peters, Methods and compositions for long fragment read sequencing ,(2010)
Serge Saxonov, Donald Masquelier, Mirna Jarosz, Kevin Ness, Andrew Price, Paul Hardenbol, Christopher Hindson, Yuan Li, Landon Merrill, Benjamin Hindson, Michael Schnall-Levin, Compositions and methods for sample processing ,(2014)