A sensitive bioluminescent immunoassay for dinitrophenol and trinitrotoluene.
作者： Jon Wannlund , Marlene Deluca
DOI: 10.1016/0003-2697(82)90300-1
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摘要: Abstract A bioluminescent immunoassay for measuring dinitrophenol and trinitrotoluene (TNT) has been developed. The DNP TNT were covalently linked to firefly luciferase, resulting in a conjugate containing 1 mol of or trinitrophenyl (TNP) per mole luciferase. retained 90% its original catalytic activity. When the was incubated with immobillzed anti-TNT anti-DNP varying concentrations free DNP-leucine, amount bound inversely proportional concentration compound. Using this procedure it is possible detect 2.5 pmol DNP-leucine 1.0 TNT. If TNP glucose-6-phosphate dehydrogenase instead much lower quantities antigen can be detected. This due fact that enzyme large turnover number so amplification possible. NADH produced measured using immobilized bacterial NADH:FMN oxidoreductase With procedure, 10 amol (10 −17 mol) measured. These procedures should suitable any antigen.
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