Differential expression of DHHC9 in microsatellite stable and instable human colorectal cancer subgroups

摘要: Microarray analysis on pooled samples has previously identified ZDHHC9 (DHHC9) to be upregulated in colon adenocarcinoma compared normal mucosa. Analyses of 168 from proximal and distal adenocarcinomas using U133plus2.0 microarrays validated these findings, showing a significant two-fold (log 2) upregulation DHHC9 transcript (P<10−6). The was more striking microsatellite stable (MSS), than instable (MSI), tumours. Genes known interact with as H-Ras or N-Ras did not show expression differences between MSS MSI. Immunohistochemical performed 60 adenocarcinomas, analysed microarrays, well tissue 40 stage I–IV tumours 46 different organ sites. protein strongly expressed MSI tumours, readily detectable premalignant lesions, the rare seen specific for gastrointestinal tract localised Golgi apparatus, vitro vivo. Overexpression decreased proliferation SW480 CaCo2 cell lines significantly. In conclusion, is gastrointestinal-related highly palmitoyl transferase activity, modifying H-Ras, suggests target anticancer drug design.