Properties and spatial arrangement of components in preinitiation complexes of eukaryotic protein synthesis.
作者： Heinz Bielka
DOI: 10.1016/S0079-6603(08)60351-7
关键词:
摘要: Publisher Summary This chapter discusses the properties and spatial arrangement of components in preinitiation complexes eukaryotic protein synthesis. The topography proteins ribosomes has been studied so far mainly by cross-linking immune electron microscopy. composition 42 cross-linked DBI within 40-S ribosomal subunit from rat liver analyzed. By this procedure, 25 out 30 contained small were identified as dimers. For S4, S20, S3, eight, seven, six neighbors found, respectively; three to five for 16 other remaining could only be one or two proteins. Topological mapping on particles is possible also using antibodies against combination with microscopy (immune microscopy). method was first applied localization E. coli principle technique involves reaction individual subsequent microscopic investigation ribosome–antibody after negative staining.
sci-hub.se 参考文章(49)
B. Gross, P. Westermann, H. Bielka, Spatial arrangement of proteins within the small subunit of rat liver ribosomes studied by cross-linking. The EMBO Journal. ,vol. 2, pp. 255- 260 ,(1983) , 10.1002/J.1460-2075.1983.TB01414.X
O. Nygård, H. Nika, Identification by RNA-protein cross-linking of ribosomal proteins located at the interface between the small and the large subunits of mammalian ribosomes. The EMBO Journal. ,vol. 1, pp. 357- 362 ,(1982) , 10.1002/J.1460-2075.1982.TB01174.X
Seiichi YOKOE, Minoru TANAKA, Hiroshige HIBASAMI, Jun NAGAI, Kunio NAKASHIMA, Cross-Linking of Tobacco Mosaic Virus RNA and Capped Polyribonucleotides to 18S rRNA in Wheat Germ Ribosome-mRNA Complexes Journal of Biochemistry. ,vol. 94, pp. 1803- 1808 ,(1983) , 10.1093/OXFORDJOURNALS.JBCHEM.A134532
Yoshiaki TAKAHASHI, Kikuo OGATA, Ribosomal proteins cross-linked to natural mRNA by UV irradiation of rat liver polysomes. Journal of Biochemistry. ,vol. 90, pp. 1549- 1552 ,(1981) , 10.1093/OXFORDJOURNALS.JBCHEM.A133624
Kazuo TERAO, Kikuo OGATA, Proteins of small subunits of rat liver ribosomes that interact with poly(U). II. Cross-links between poly(U) and ribosomal proteins in 40 S subunits induced by UV irradiation. Journal of Biochemistry. ,vol. 86, pp. 605- 617 ,(1979) , 10.1093/OXFORDJOURNALS.JBCHEM.A132564
Brian Safer, 2B or not 2B: Regulation of the catalytic utilization of elF-2 Cell. ,vol. 33, pp. 7- 8 ,(1983) , 10.1016/0092-8674(83)90326-4
J. A. Lake, M. Pendergast, L. Kahan, M. Nomura, Localization of Escherichia coli Ribosomal Proteins S4 and S14 by Electron Microscopy of Antibody-Labeled Subunits Proceedings of the National Academy of Sciences of the United States of America. ,vol. 71, pp. 4688- 4692 ,(1974) , 10.1073/PNAS.71.12.4688
Georgia A. FLOYD, Jolinda A. TRAUGH, William C. MERRICK, Identification of Initiation Factors and Ribosome‐Associated Phosphoproteins by Two‐Dimensional Polyacrylamide Gel Electrophoresis FEBS Journal. ,vol. 96, pp. 277- 286 ,(1979) , 10.1111/J.1432-1033.1979.TB13038.X
Odd Nygård, Peter Westermann, Tore Hultin, The use of rRNA-cellulose chromatography in the rapid isolation of homogeneous protein synthesis initiation factor eIF-2 from rat liver microsomes Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. ,vol. 608, pp. 196- 200 ,(1980) , 10.1016/0005-2787(80)90148-3
Peter Westermann, Odd Nygārd, Heinz Bielka, The alpha and gamma subunits of initiation factor eIF-2 can be cross-linked to 18S ribosomal RNA within the quaternary initiation complex, eIF-2.Met-tRNAf.GDPCP.small ribosomal subunit. Nucleic Acids Research. ,vol. 8, pp. 3065- 3072 ,(1980) , 10.1093/NAR/8.14.3065