Identification by RNA-protein cross-linking of ribosomal proteins located at the interface between the small and the large subunits of mammalian ribosomes.

摘要: Abstract Protein constituents at the subunit interface of rat liver ribosomes were analysed by cross-linking with bifunctional reagent, diepoxybutane (distance between reactive groups 4 A). Isolated 40S and 60S subunits labelled 125I recombined unlabelled complementary subunits. The two kinds selectively 80S treated low concentration. Radioactive ribosomal proteins covalently attached to rRNA subparticles isolated repeated gradient centrifugation. RNA-bound, identified two-dimensional gel electrophoresis. experiments showed that S2, S3, S4, S6, S7, S13, S14 in small are located close proximity 28S rRNA. Similarly, L3, L6, L7, L8 found large vicinity 18S