Co-entrapment of Trichoderma harzianum and Glomus sp. within alginate beads: impact on the arbuscular mycorrhizal fungi life cycle.

作者: N. De Jaeger , I.E. de la Providencia , H. Rouhier , S. Declerck

DOI: 10.1111/J.1365-2672.2011.05035.X

关键词:

摘要: Aims:  This study was performed to explore the compatibility and applicability of plant beneficial micro-organisms (i.e. Trichoderma harzianum MUCL 29707 Glomus sp. 41833) co-entrapped in alginate beads. Methods Results:  Spores conidia T. were immobilized beads impacts saprotrophic fungi on presymbiotic symbiotic phase arbuscular mycorrhizal (AM) evaluated under strict vitro culture conditions. Our results demonstrated capacity both combination regrowth outside calcium coating. The presence did not hinder AM fungal development but rather stimulated its spore production fitness. Conclusions:  with 41833 may represent a reliable alternative inoculum formulation for application sustainable agriculture. Significance Impact Study:  entrapment two saprotroph symbiont) having effects plants represents promising inoculants adapted field application.

参考文章(39)
Sylvie Cranenbrouck, Liesbeth Voets, Céline Bivort, Laurent Renard, Désiré-Georges Strullu, Stéphane Declerck, Methodologies for in Vitro Cultivation of Arbuscular Mycorrhizal Fungi with Root Organs Springer, Berlin, Heidelberg. pp. 341- 375 ,(2005) , 10.1007/3-540-27331-X_18
M. Giovannetti, Survival Strategies in Arbuscular Mycorrhizal Symbionts Cellular Origin, Life in Extreme Habitats and Astrobiology. pp. 293- 307 ,(2001) , 10.1007/0-306-48173-1_18
N. De Jaeger, C. Decock, S. Declerck, I. E. De la Providencia, Coupling autotrophic in vitro plant cultivation system to scanning electron microscope to study plant-fungal interactions Spanish Journal of Agricultural Research. ,vol. 8, pp. 69- 78 ,(2010) , 10.5424/SJAR/201008S1-1224
Landis W. Doner, Guillaume Bécard, SOLUBILIZATION OF GELLAN GELS BY CHELATION OF CATIONS Biotechnology Techniques. ,vol. 5, pp. 25- 28 ,(1991) , 10.1007/BF00152749
Liesbeth Voets, Hervé Dupré de Boulois, Laurent Renard, Désiré-Georges Strullu, Stéphane Declerck, Development of an autotrophic culture system for the in vitro mycorrhization of potato plantlets Fems Microbiology Letters. ,vol. 248, pp. 111- 118 ,(2005) , 10.1016/J.FEMSLE.2005.05.025
B. Tisserant, V. Gianinazzi-Pearson, S. Gianinazzi, A. Gollotte, In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections Fungal Biology. ,vol. 97, pp. 245- 250 ,(1993) , 10.1016/S0953-7562(09)80248-7
A. Hodge, T. Helgason, A.H. Fitter, Nutritional ecology of arbuscular mycorrhizal fungi Fungal Ecology. ,vol. 3, pp. 267- 273 ,(2010) , 10.1016/J.FUNECO.2010.02.002
Jean Weber, Marc Ducousso, Foong Yee Tham, Sophie Nourissier-Mountou, Antoine Galiana, Yves Prin, Sing Kong Lee, Co-inoculation of Acacia mangium with Glomus intraradices and Bradyrhizobium sp. in aeroponic culture Biology and Fertility of Soils. ,vol. 41, pp. 233- 239 ,(2005) , 10.1007/S00374-005-0833-Z