Viral determinants of rotavirus pathogenicity in pigs: production of reassortants by asynchronous coinfection.

摘要: A porcine rotavirus (prv), variant 4F, isolated in tissue culture from the feces of a Chinese pig with diarrhea, was found to have become highly pathogenic when passaged gnotobiotic piglets (J. C. Bridger, B. Burke, G. M. Beards, and U. Desselberger, J. Gen. Virol. 73:3011-3015, 1992). Comparison closely related pig-apathogenic prv 4S suggested outer capsid protein VP4 (encoded by RNA 4) 4F as determinant for pathogenicity (B. 75:2205-2212, 1994; Virology 202:754-759, 1994). In order provide more direct evidence, which grows forms plaques poorly reassorted well-tissue-culture-adapted, bovine (brv; UK Compton strain). After asynchronous coinfection cell cultures (first followed brv 6 12 h later), several reassortants were containing 4 either alone (isolate B-F4) or together one two other genes genetic background brv. Backcrossing monoreassortant B-F4 yielded monoreassortant, S-F4, carries 4S. The vitro growth characteristics these analyzed, roles plaque formation kinetics confirmed. S-F4 parental viruses are currently being tested Tauscher, unpublished data).