Phenotypes of rotavirus reassortants depend upon the recipient genetic background

摘要: Abstract We have previously characterized the biological and immunological properties of a simian rotavirus SA11 variant (4F) with an altered genome segment 4. The SA11-4F formed large plaques in presence protease, small clear absence grew to high titer protease when compared our standard wild type (SA11 clone 3). To determine 4F that encoded unique protease-associated phenotypes variant, reassortants were generated segregated outer capsid genes onto genetic background derived from either bovine B223 or (clone 3), both which contrasting phenotypes. parental reassortant viruses examined ability (i) form (ii) exogenous (iii) grow significantly higher protease. These could be transferred 3 by single However, 4F/B223 different unexpected situation was found. On same combination minimum two segments, segments 4 9. results indicate recipient donor are reassorted can affect conferred segments. Thus, segregation mapping experiments using should interpreted caution.