Measurement of reoxygenation during fractionated radiotherapy in head and neck squamous cell carcinoma xenografts

摘要: Hypoxic tissues lack adequate oxygenation and it has been long established that tumours commonly exhibit hypoxia is a factor contributing towards resistance to radiotherapy. To develop computer models make predictions about the affects of tumour on treatment outcome, quantitative reoxygenation data from in vivo systems required. The aim this study was investigate timing degree during radiotherapy human head neck squamous cell carcinoma xenograft mouse model (FaDu). Mice were immobilised using novel restraining system exposed unanaesthetised 3 or 5 Gy fractions, up maximum 40 Gy. Partial pressures oxygen (pO2) measurements recorded at six time points throughout 2 week course radiotherapy, fibre optic system. Tumours receiving 0–30 did not an increase pO2. However, mean pO2 after weeks accelerated fractionated (40 Gy) significantly increased (P < 0.01) compared full schedule (0–30 Gy). These results lead conclusion average onset group xenografts. A relatively large range values measured each dose point indicate inter-tumour variation among tumours. Data experimental work will be used define times implemented Monte Carlo model, simulating hypoxic cancer growth