Two-Beam Cross-Correlation: A Method To Characterize Transport Phenomena in Micrometer-Sized Structures

摘要: To determine flow properties, namely, the velocity and angle of in microstructured channels, an experimental realization based on fluorescence correlation spectroscopy is described. For this purpose, two micrometer-sized spatially separated volume elements have been created. The cross-correlation signal from these has recorded evaluated mathematically. In addition to previous results, two-beam allows for fast easy determination even small (down 200 μm/s) velocities, as well simultaneous measurement diffusion properties single dye molecules within a rather short detection time 5−100 s error rate less than 20%. spatial resolution around 1−2 μm, limited by diameter element. Furthermore, vectorial data can be obtained evaluated. A discussion theoretical background verification results performed. feasibility processing show...