Streptomyces glucose/xylose isomerase has a single active site for glucose and xylose

作者: Sushama M. Gaikwad , Hemant S. Pawar , Hari G. Vartak , Vasanti V. Deshpande

DOI: 10.1016/0006-291X(89)90014-4

关键词:

摘要: A kinetic method which allows one to evaluate whether an enzyme acting on two different substrates has or active sites was employed study the site of glucose isomerase catalyses isomerization both and xylose. The experimental data rates hydrolysis mixtures various concentrations xylose by from Streptomyces coincides well with theoretical values calculated for case a single site.

参考文章(9)
Zacharias. Dische, Ellen. Borenfreund, A NEW SPECTROPHOTOMETRIC METHOD FOR THE DETECTION AND DETERMINATION OF KETO SUGARS AND TRIOSES Journal of Biological Chemistry. ,vol. 192, pp. 583- 587 ,(1951) , 10.1016/S0021-9258(19)77782-5
R. O. MARSHALL, E. R. KOOI, G. M. Moffett, Enzymatic conversion of D-glucose to D-fructose. Science. ,vol. 125, pp. 648- 649 ,(1957) , 10.1126/SCIENCE.125.3249.648
H. G. Vartak, M. C. Srinivasan, V. K. Powar, M. V. Rele, J. M. Khire, Characterization of extracellular substrate specific glucose and xylose isomerases of Chainia Biotechnology Letters. ,vol. 6, pp. 493- 494 ,(1984) , 10.1007/BF00139990
M. Callens, H. Kerster-Hilderson, O. Van Opstal, C. K. De Bruyne, D-xylose isomerase from Streptomyces violaceus-ruber: optimization of fermentation conditions Biotechnology Letters. ,vol. 7, pp. 597- 600 ,(1985) , 10.1007/BF01026456
Irwin A. Rose, Edward L. O'Connell, Intramolecular hydrogen transfer in the phosphoglucose isomerase reaction. Journal of Biological Chemistry. ,vol. 236, pp. 3086- 3092 ,(1961) , 10.1016/S0021-9258(18)93975-X
Yoshiyuki TAKASAKI, Osamu TANABE, Studies on Isomerization of Sugars by Bacteria Part IX Agricultural and biological chemistry. ,vol. 30, pp. 220- 225 ,(1966) , 10.1271/BBB1961.30.220
W Vangrysperre, M Callens, H Kersters-Hilderson, C K De Bruyne, Evidence for an essential histidine residue in D-xylose isomerases. Biochemical Journal. ,vol. 250, pp. 153- 160 ,(1988) , 10.1042/BJ2500153