Molt stage and cuticle damage influence white spot syndrome virus immersion infection in penaeid shrimp.

摘要: Transmission of white spot syndrome virus (WSSV) in shrimp has been reported to occur by feeding and immersion. In the present study, impact molt process artificial lesions cuticle on susceptibility WSSV was examined using intramuscular immersion routes. For route, Penaeus (Litopenaeus) vannamei (n=450) were injected with 10(-2.3) up 10(2.7) infectious dose 50% end point (SID(50)) early late post-molt, inter-molt, pre-molt; resp. A-, B-, C-, D1- D2-stage. The resulting infection titers demonstrated that no difference (p>0.05) existed between different stages when injected. waterborne immersed seawater containing 10(4)SID(50)ml(-1) WSSV. a first P. (n=125) incubated cell culture flasks, became infected mostly post-molt stages. second 2 groups (n=100) monodon transferred into plastic bags prevent damage cuticle; 1 group pleopod cut off prior incubation. Induction increased significantly (p<0.05) A-stage from 0-40% 60-100%, B-stage 0-20% 40-60%, C-stage 0-20 20-60%, while 0% D-stages both methods. This study proved are more susceptible via after molting than period before wounding facilitates infection.