7-Ketocholesterol Promotes Oxiapoptophagy in Bone Marrow Mesenchymal Stem Cell from Patients with Acute Myeloid Leukemia

摘要: 7-Ketocholesterol (7-KC) is a cholesterol oxidation product with several biological functions. 7-KC has the capacity to cause cell death depending on concentration and specific type. Mesenchymal stem cells (MSCs) are multipotent ability differentiate into various types of cells, such as osteoblasts adipocytes, among others. MSCs contribute development suitable niche for hematopoietic involved in diseases, leukemia, yet unknown extent. Here, we describe effect bone marrow from patients acute myeloid leukemia (LMSCs). LMSCs were less susceptible death-promoting than other types. exposure triggered extrinsic pathway apoptosis an increase activated caspase-8 caspase-3 activity. Mechanisms caspase-dependent pathways involved. increased ROS generation by LMSCs, which was related decreased viability. also led disruption cytoskeleton number S phase, G1/S transition. Autophagosome accumulation observed. downregulated SHh protein but did not change expression SMO. In conclusion, oxiapoptophagy (OXIdative stress + APOPTOsis autophagy) seems be LMSCs. More studies needed better understand role possible effects pathway.