Occurrence of a novel NADP(+)-linked alcohol dehydrogenase in Euglena gracilis.

摘要: An NADP(+)-dependent alcohol dehydrogenase was found in Euglena gracilis Z grown on 1-hexanol, while it detected at low activity cells ethanol or glucose as a carbon source, indicating that the enzyme is induced by addition of 1-hexanol into medium source. This extremely unstable, even 4 degrees C, unless 20% ethylene glycol added. The optimal pH 8.8-9.0 for oxidation reaction. apparent K(m) values and NADP(+) were to be 6.79 mM 46.7 microM this enzyme, respectively. substrate specificity very different from already purified NAD(+)-specific showing highest with substrate, followed 1-pentanol 1-butanol, there little 1-propanol. active towards primary alcohols but not secondary alcohols. Accordingly, since NADP(+)-specific separated DEAE cellulose column, confirmed contain novel middle long-chain length fatty