Glycine Uptake in Escherichia coli: II. GLYCINE UPTAKE, EXCHANGE, AND METABOLISM BY AN ISOLATED MEMBRANE PREPARATION

摘要: Abstract In this paper, glycine uptake by isolated membrane preparations of Escherichia coli W (W+) and the d-serine-resistant mutant (WS) is examined in detail. Glycine W+ dependent on pH presence Mg++ an energy source. membranes, first taken up into exchangeable pool, from which it subsequently removed as incubation proceeds. The pool built within 30 sec remains constant with time, although membranes continues. independent temperature. Uptake exchange are specific for glycine, dl-alanine, dl-serine, dl-threonine. Membrane WS unable to catalyze either or between medium pool. inhibited 50 70% anaerobiosis, at 0°, 2,4-dinitrophenol, carbonyl cyanide p-trifluoromethoxyphenylhydrazone, NaN3, KAsO4, KCN, iodoacetamide, p-chloromercuriphenylsulfonate. No inhibition oberved NaF, sodium Amytal, hydroxyquinoline N-oxide, antimycin A, Chloromycetin, ouabain. Although min conditions listed above, there no diminution when measured sec. Under none studied (at min) decreased values found 37° 0°. incubated convert about 94% carbon compounds that have properties phosphatidylethanolamine phosphatidylserine. all studied, concentration intramembranal approximates medium. Incubation 0° dinitrophenol iodoacetamide inhibits conversion phospholipids, amount increased above uninhibited (control) preparations. significant amounts but only 10 20% external When raised so rate comparable exhibited much phospholipids do Hydroxylamine almost quantitatively inhibitor manifest same defect.