Transformation of Mouse Macrophages by Simian Virus 40

摘要: Abstract Studies were undertaken to prove that simian virus 40 (SV40) can transform the mouse macrophage, a cell type naturally restricted from deoxyribonucleic acid (DNA) replication. Balb/C macrophages infected with SV40 demonstrated T-antigen production and induced DNA synthesis simultaneously. In absence of apparent division, these cells remained T antigen-positive for at least 45 days. could be rescued nondividing, unaltered during antigen-producing period. Proliferating transformants appeared an average 66 days post-SV40 infection. Established lines negative infectious virus, but yielded after fusion African green monkey kidney cells. Their identity as transformed was substantiated by evaluation cellular morphology, phagocytosis, phosphatase, β1c synthesis, aminoacridine incorporation.