Add-on plasmonic patch as a universal fluorescence enhancer.

摘要: Fluorescence-based techniques are the cornerstone of modern biomedical optics, with applications ranging from bioimaging at various scales (organelle to organism) detection and quantification a wide variety biological species interest. However, weakness fluorescence signal remains persistent challenge in meeting ever-increasing demand image, detect, quantify low abundance. Here, we report simple universal method based on flexible conformal elastomeric film adsorbed plasmonic nanostructures, which term “plasmonic patch,” that provides large (up 100-fold) uniform enhancement surfaces through transfer patch surface. We demonstrate improving sensitivity limit (by more than 100 times) fluorescence-based immunoassays implemented microtiter plates microarray format. The novel approach presented here represents disease, biomarker, application agnostic ubiquitously applicable fundamental enabling technology immediately improve existing analytical methodologies an easy-to-handle cost-effective manner, without changing original procedures techniques.