Phenylpropanoid production in callus and cell suspension cultures of Buddleja cordata Kunth

作者: M. E. Estrada-Zúñiga , F. Cruz-Sosa , M. Rodríguez-Monroy , J. R. Verde-Calvo , E. J. Vernon-Carter

DOI: 10.1007/S11240-009-9496-Z

关键词:

摘要: Plant tissue cultures represent a potential source for producing secondary metabolites. In this work, Buddleja cordata were established in order to produce phenylpropanoids (verbascoside, linarin and hydroxycinnamic acids), as these metabolites are credited with therapeutic properties. Highest callus induction (76.4–84.3%) was obtained five treatments containing 2,4-Dichlorophenoxyacetic acid (2,4-d: 0.45–9.05 μM) Kinetin (KIN: 2.32, 4.65 μM), whereas highest root (79.6%) corresponded the α-Naphthaleneacetic (9.05 KIN (2.32 treatment. Verbascoside major phenylpropanoid produced vitro (root, white green callus) [66.24–86.26 mg g−1 dry weight (DW)], while production low (0.95–3.01 DW). improved cell suspension culture (116 DW 8.12 DW, respectively).

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