Galactomannan-PEI based non-viral vectors for targeted delivery of plasmid to macrophages and hepatocytes

摘要: Intracellular nature and diversified locations of infectious parasitic diseases such as leishmaniasis, trypanosomiasis, tuberculosis hepatitis B C pose a significant global burden challenge to the scientists working in area drug discovery delivery. The macrophages hepatocytes are considered potential target sites they together play an important role various diseases. present study scrutinizes applicability natural biopolymer-based chemical vectors, capable targeting both hepatocytes, that can form complex with plasmid administer it into cells produce desired protein. investigations were made develop novel series gene carriers by conjugating depolymerized galactomannan (guar gum), biocompatible polysaccharide low molecular weight branched PEI (LMWP). A conjugates developed characterized using physicochemical techniques. All GP/pDNA complexes showed significantly higher transfection efficiency GP-3/pDNA, one best formulations, ~2.0-7.7-folds efficacy when compared standard reagents. Further, GP-3/pDNA displayed specific under vitro vivo conditions. data demonstrate GP vectors deliver nucleic acids simultaneously delivery applications.