Retinoblastoma-binding protein 2 (RBP2) is frequently expressed in neuroendocrine tumors and promotes the neoplastic phenotype

摘要: Neuroendocrine tumors (NETs), which can have survival rates as low 4%, currently limited therapeutic interventions available highlighting the dire need for identification of novel biological targets use new potential drug targets. One such target is retinoblastoma-binding protein 2 (RBP2), an H3K4 demethylase whose overexpression has been linked to cancer formation and metastasis in non-endocrine tumor types. We measured RBP2 mRNA levels enteropancreatic NETs by measuring matched human normal NET tissue samples. Further, proliferation, migration, invasion colony assays were performed physiologically relevant cell lines βlox5, H727 QGP-1 understand role its activity on end points tumorigenesis. Our data indicate a strong correlation between expression specimens. was overexpressed relative tissue-matched controls 80% measured. In vitro studies showed significantly increased formation, whereas knockdown decreases same parameters demethylase-independent manner. The cycle inhibitors p21 p57 decreased with upon depletion, suggesting regulatory cellular proliferation. Taken together, our results support hypothesis that aberrant frequent contributing factor NETs.