Enhanced synthesis de novo of phosphatidylinositol in lymphocytes treated with cationic amphiphilic drugs.

摘要: A variety of amphiphilic cations caused very large increases in the rates incorporation Pi and glycerol into phosphatidylinositol pig mesenteric small lymphocytes. This synthesis de novo led to a doubling concentration cells within 3.5 h. The increase labelled with [3H]- or [14C]-glycerol was matched by an approximately equivalent decrease phosphatidylcholine, phosphatidylethanolamine triacylglycerol. Amphilic which produced these effects included, order decreasing effectiveness, trifluoperazine (half-maximal effect at about 70 mum) greater than chlorpromazine promethazine imipramine cinchocaine amethocaine cetyltrimethylammonium fenfluramine amphetamine 2-phenethylamine cocaine procaine; most effective compounds were those largest hydrophobic non-polar substituents. response not changed varying extracellular Ca2+ range 10 nm-1mm. active interacted anionic phospholipids causing aggregation aqueous dispersions and/or changes chromatographic behaviour. These results indicate that redirect glycerolipid novo, probably owing inhibition phosphatidate phosphohydrolase, so is increased expense other glycerolipids.