Biomechanical Strain Induces Class A Scavenger Receptor Expression in Human Monocyte/Macrophages and THP-1 Cells A Potential Mechanism of Increased Atherosclerosis in Hypertension

摘要: Background—Although hypertension is an important risk factor for the development of atherosclerosis, mechanisms this interaction are incompletely described. Previous studies have suggested that biomechanical strain regulates macrophage phenotype. We tested hypothesis can induce expression class A scavenger receptor (SRA), lipoprotein in atherogenesis. Methods and Results—Human monocyte/macrophages or THP-1 cells were cultured a device imposes uniform biaxial cyclic 1-Hz strains 0%, 1%, 2%, 3%, SRA was analyzed. Mechanical induced mRNA (3.5±0.6-fold at 3% 48 hours, P<0.01) protein amplitude-dependent manner. This induction accompanied by augmented B CD36 (2.8±0.3-fold, P<0.001) but not increased peroxisome proliferator–activated receptor-γ expression. To evaluate effect vivo, apolipoprotein E−/− mice wer...