The role of molybdenum in formation of the NADPH-nitrate reductase by

作者: R.J. Downey

DOI: 10.1016/0006-291X(73)91333-8

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摘要: Abstract Non-nitrate reducing mutants of Aspergillus nidulans have been noted to produce either a nitrate inducible or constitutive NADPH-cytochrome c reductase which resides in 4.5s 7.8s protein. The latter closely resembles the inducible, FAD dependent NADPH-nitrate from wild type. Measurement flavin adenine dinucleotide (FAD) and molybdenum (Mo) these two proteins revealed significant differences particularly Mo. concepts that nia gene product constitutes major bearing component enzyme constitutively produced cnx is implicated formation larger Mo multimer are further supported.

参考文章(12)
Chester DeLuca, Morton M. Weber, Nathan O. Kaplan, A specific spectrophotometric assay for flavin adenine dinucleotide. Journal of Biological Chemistry. ,vol. 223, pp. 559- 567 ,(1956) , 10.1016/S0021-9258(18)65164-6
Helen B. Burch, Otto A. Bessey, Oliver H. Lowry, Fluorometric measurements of riboflavin and its natural derivatives in small quantities of blood serum and cells. Journal of Biological Chemistry. ,vol. 175, pp. 457- 470 ,(1948) , 10.1016/S0021-9258(18)57276-8
D. J. Cove, J. A. Pateman, Autoregulation of the Synthesis of Nitrate Reductase in Aspergillus nidulans Journal of Bacteriology. ,vol. 97, pp. 1374- 1378 ,(1969) , 10.1128/JB.97.3.1374-1378.1969
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
John L. Wray, Philip Filner, Structural and functional relationships of enzyme activities induced by nitrate in barley Biochemical Journal. ,vol. 119, pp. 715- 725 ,(1970) , 10.1042/BJ1190715
C. M. Johnson, T. H. Arkley, Determination of Molybdenum in Plant Tissue Analytical Chemistry. ,vol. 26, pp. 572- 574 ,(1954) , 10.1021/AC60087A048
J. A. PATEMAN, D. J. COVE, B. M. REVER, D. B. ROBERTS, A Common Co-Factor for Nitrate Reductase and Xanthine Dehydrogenase which also Regulates the Synthesis of Nitrate Reductase Nature. ,vol. 201, pp. 58- 60 ,(1964) , 10.1038/201058A0
JA Pateman, BM Rever, DJ Cove, Genetic and biochemical studies of nitrate reduction in Aspergillus nidulans Biochemical Journal. ,vol. 104, pp. 103- 111 ,(1967) , 10.1042/BJ1040103