Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21)
作者： Géraldine Nollevaux , Christelle Devillé , Benaïssa El Moualij , Willy Zorzi , Patricia Deloyer
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摘要: The absorptive and goblet cells are the main cellular types encountered in intestine epithelium. cell lineage Caco-2 is a model commonly used to reproduce features of bowel However, there strong debate regarding value culture mimick vivo situation. Indeed, some authors report low paracellular permeability an ease access highly diffusible small molecules microvilli, due almost complete lack mucus. HT29-5M21 intestinal mucin-secreting population. A co-culture system carried out serum-free medium comprising both was developed. systematic use requires characterization monolayer under given experimental procedure. In this study, we investigated activity localization alkaline phosphatase expression IAP MUC5AC genes determine correlation between these markers composition differentiated obtained from mixture cells. We observed that conditions (serum-free medium) did not change phenotype each type, produced reproducible model. characterizing influenced by presence formed 75% 25% produce containing two human epithelium characterized reduced macromolecules.
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