Technology and method to study microbial growth and adhesion to host-related surface and the host-microbiota interactions
作者： Pieter Van Den Abbeele , Massimo Marzorati , Barbara Vanhoecke , Willy Verstraete , Sam Possemiers
DOI:
关键词:
摘要: The present invention relates to in vitro adhesion modules that allow growth, stabilization and study of microbial communities adhere colonize host- related surfaces, mimic transport chemical compounds across epithelial surfaces simulate host-microorganism interactions adaptation. It includes the provision micromolar amounts oxygen via basal side a mucus layer towards adhered microorganisms thus establishing microaerophilic conditions prevailing at base biofϊlm. can also include cells, simulating host, chamber on functional comprising said layer. module be placed between different compartments SHIME - Simulator Human Intestinal Microbial Ecosystem. An extension is made where duodenum, jejunum ileum are separately mimicked.
lens.org 参考文章(33)
Martin L. Yarmush, Paul A. Dimilla, Mehmet Toner, Maury D. Cosman, Ulysses J. Balis, Arno W. Tilles, Cell culture systems and methods for organ assist devices ,(2000)
Ismail Fliss, Christophe Lacroix, Gwenaelle LeBlay, Cecile Cinquin, In vitro gastrointestinal model system and uses thereof ,(2003)
K Pedersen, G W Tannock, Colonization of the porcine gastrointestinal tract by lactobacilli. Applied and Environmental Microbiology. ,vol. 55, pp. 279- 283 ,(1989) , 10.1128/AEM.55.2.279-283.1989
Géraldine Nollevaux, Christelle Devillé, Benaïssa El Moualij, Willy Zorzi, Patricia Deloyer, Yves-Jacques Schneider, Olivier Peulen, Guy Dandrifosse, Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21) BMC Cell Biology. ,vol. 7, pp. 20- 20 ,(2006) , 10.1186/1471-2121-7-20
Stephen G. Emerson, Richard M. Schwartz, Bernhard O. Palsson, Devices for maintaining and growing human stem and/or hematopoietics cells ,(1994)
A. Parlesak, D. Haller, S. Brinz, A. Baeuerlein, C. Bode, Modulation of Cytokine Release by Differentiated CACO‐2 Cells in a Compartmentalized Coculture Model with Mononuclear Leucocytes and Nonpathogenic Bacteria Scandinavian Journal of Immunology. ,vol. 60, pp. 477- 485 ,(2004) , 10.1111/J.0300-9475.2004.01495.X
H. M. Probert, G. R. Gibson, Development of a fermentation system to model sessile bacterial populations in the human colon Biofilms. ,vol. 1, pp. 13- 19 ,(2004) , 10.1017/S1479050503001029
Mark M Huycke, Danny R Moore, In vivo production of hydroxyl radical by enterococcus faecalis colonizing the intestinal tract using aromatic hydroxylation Free Radical Biology and Medicine. ,vol. 33, pp. 818- 826 ,(2002) , 10.1016/S0891-5849(02)00977-2
Sarah E. Cheesman, Karen Guillemin, We know you are in there: conversing with the indigenous gut microbiota. Research in Microbiology. ,vol. 158, pp. 2- 9 ,(2007) , 10.1016/J.RESMIC.2006.10.005
Timothy M. Straub, Kerstin Höner zu Bentrup, Patricia Orosz Coghlan, Alice Dohnalkova, Brooke K. Mayer, Rachel A. Bartholomew, Catherine O. Valdez, Cynthia J. Bruckner-Lea, Charles P. Gerba, Morteza A. Abbaszadegan, Cheryl A. Nickerson, In vitro cell culture infectivity assay for human noroviruses. Emerging Infectious Diseases. ,vol. 13, pp. 396- 403 ,(2007) , 10.3201/EID1303.060549