Cytotoxic and genotoxic effects of areca nut-related compounds in cultured human buccal epithelial cells.

摘要: Abstract Because betel quid chewing has been linked to the development of oral cancer, pathobiological effects an aqueous areca nut extract, four alkaloids (arecoline, guvacoline, guvacine, and arecaidine), nitrosated derivatives [N-nitrosoguvacoline, N-nitrosoguvacine, 3-(N-nitrosomethylamino)propionaldehyde 3-(N-nitrosomethylamino)propionitrile] have investigated using cultured human buccal epithelial cells. Areca extract in a dose-dependent manner decreases cell survival, vital dye accumulation, membrane integrity, it causes formation both DNA single strand breaks protein cross-links. Depletion cellular free low-molecular-weight thiols also occurs, albeit at quite toxic concentrations. Comparisons nut-related N-nitroso compounds their precursor alkaloids, concentrations up 5 mm, indicate that is most potent on molar basis decrease survival thiol content cause significant breaks. Arecoline, or N-nitrosoguvacoline thiols, whereas arecaidine, 3-(N-nitrosomethylamino)propionitrile only minor these variables. Taken together, present studies and, particular, one compound related nut, i.e., 3-(N-nitrosomethylamino)propionaldehyde, are highly cytotoxic genotoxic cells, potential importance induction tumors chewers.