A multicomponent complex is involved in the splicing of messenger RNA precursors
作者： P GRABOWSKI , S SEILER , P SHARP
DOI: 10.1016/S0092-8674(85)80130-6
关键词:
摘要: A multicomponent complex termed spliceosome (splicing body) is unique to the splicing of messenger RNA precursors in vitro. This 60S RNA-protein contains RNAs from previously characterized bipartite intermediate, 5' exon RNA, and lariat intervening sequence-3' as well some intact 455 nucleotide precursor RNA. snRNPs, particularly U1 RNP, shown by immunoprecipitation with specific antisera. Formation appears be an early essential step splicing, because forms during stage, or lag time, reaction before first covalent modification, cleavage at splice site The only under conditions that permit splicing; both ATP a containing authentic 3' sites are required for formation, while antiserum RNP inhibits its formation. within complex, predominantly was chased into products accelerated kinetics more complete conversion than purified
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