Cloning and characterization of AtRGP1. A reversibly autoglycosylated arabidopsis protein implicated in cell wall biosynthesis.
作者： Ivan J. Delgado , Zhaohong Wang , Amy de Rocher , Kenneth Keegstra , Natasha V. Raikhel
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摘要: A reversibly glycosylated polypeptide from pea ( Pisum sativum ) is thought to have a role in the biosynthesis of hemicellulosic polysaccharides. We investigated this hypothesis by isolating cDNA clone encoding homolog rabidopsis t haliana, Reversibly GlycosylatedPolypeptide-1 (AtRGP1), and preparing antibodies against protein encoded gene. Polyclonal detect homologs both dicot monocot species. The patterns expression intracellular localization were examined. AtRGP1 RNA concentration are highest roots suspension-cultured cells. Localization shows it be mostly soluble but also peripherally associated with membranes. confirmed that produced Escherichia coli could using UDP-glucose UDP-galactose as substrates. Possible sites for UDP-sugar binding glycosylation discussed. Our results consistent cell wall biosynthesis, although its precise still unknown.
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