TMEM16A calcium-activated chloride currents in supporting cells of the mouse olfactory epithelium.

摘要: Glial-like supporting (or sustentacular) cells are important constituents of the olfactory epithelium that involved in several physiological processes such as production endocannabinoids, insulin, and ATP regulation ionic composition mucus layer covers apical surface epithelium. Supporting express metabotropic P2Y purinergic receptors generate ATP-induced Ca 2+ signaling through activation a PLC-mediated cascade. Recently, we reported subpopulation expresses also -activated Cl − channel TMEM16A. Here, sought to extend our understanding possible role this system by asking whether can activate currents mediated We use whole-cell patch-clamp analysis slices measure dose–response relations presence various intracellular concentrations, ion selectivity, blockage. find knockout TMEM16A abolishes currents, demonstrating is essential for these cells. Also, using extracellular stimuli, found stimulation activates large TMEM16A-dependent current, indicating ATP-mediated signaling. Altogether, results establish TMEM16A-mediated functional provide foundation future work investigating precise system.