Optical formation and manipulation of particle and cell patterns using a tapered optical fiber

摘要: Abstract A method for optical formation and controllable ma-nipulation of particle cell patterns using a tapered opti-cal fiber is demonstrated. With laser beam at 980-nm wave-lengthlaunchedintothefiber,differentsizedsilicaparticleswereformed into (both one-dimensional chains andtwo-dimensional arrays) with different numbers by op-tical binding. The formed can be controllablymanipulated in three dimensions. Using yeast cells as an ex-ample, it was demonstrated that the applicable forthe biological patterns, without damage to theyeast viability. This provides new facile way forbiophotonic researches particles cellsin highly organized manner. Optical manipulation particleand Hongbao Xin, Rui Xu, Baojun Li ∗ 1. Introduction Great attention has been paid recently on developmentof practical methods precise positioning arrange-ment microparticles patterns,because its great importance wide range appli-cations, such biosensor [1,2], tissue engineering [3–5],and genetic [6, 7]. Patterning methods, suchas [8], dielectrophoresis [9], acoustictweezers [10, 11], have achieved fruitful results. Amongthem, merits high flexibility, tar-geting, non-invasiveness [8]. In anemergent phenomenon Unlike opticaltweezers, binding, there are no predefinedthree-dimensional stable positions trapping andbinding results from modification opticalfield (focusing and/or diverging) presence parti-cles [12,13]. bind (1D) chains. To date, binding beenrealized various counter-propagatinglight beams [14–16] dual-beam system [17,18],forming One-, two-, three-dimensionaloptical also reportedusing two counter-propagating light [19]. addi-tion, cells[20]. Generally, bound together variousinter-particle separations several microns severaltens microns. However, number abound chain often very limited. Here we report