Immobilization of β-galactosidase on surface modified cobalt/multiwalled carbon nanotube nanocomposite improves enzyme stability and resistance to inhibitor
作者： Maryam Khan , Qayyum Husain , Rani Bushra
DOI: 10.1016/J.IJBIOMAC.2017.07.088
关键词:
摘要: The present study aimed to work out a high yield procedure for immobilization of Aspergillus oryzae β-galactosidase on polyaniline cobalt multiwalled carbon nanotubes nanocomposite (PANI/Co/MWCNTNC) by physical adsorption and covalent attachment via glutaraldehyde. binding was confirmed scanning transmission electron microscopy along with Fourier transform-infrared spectroscopy. yields obtained adsorbed cross-linked enzymes were 93% 97%, respectively. covalently immobilized enzyme remarkably more stable at extremes pH temperature compared native enzymes. Ki values found increase ten-folds indicating that this markedly resistant galactose inhibition. attached retained 92% activity after its 10th successive reuse the which exhibited 74% initial activity. Furthermore, no significant change noticed in band intensity pBR322 plasmid when exposed free bound nanocomposite. superiority terms stability reusability β-galactosidase. results, presented here demonstrate an efficient method PANI/Co/MWCNTNC order construct convenient novel biosensor detection lactose concentration.
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