12 - Murine and Guinea Pig Models of Tuberculosis

摘要: Publisher Summary This chapter reviews murine and guinea pig models of tuberculosis. Mice are the most widely used small animal model because broad availability immunological reagents, relatively low cost inbred genetically engineered strains with well-defined genotypes. The one notable disadvantage mouse is that pulmonary extra-pulmonary pathology following aerosol challenge lacks important morphologic features commonly seen in humans untreated To these aspects low-dose infection developed it shares clinical human disease. Further, highlights measuring cellular response. Measuring immune response to mycobacterial infections now common place. It describes simple methods detect responses by real-time polymerase chain reaction (RT-PCR), various ELISA-based flow cytometry.