Diatom-derived oxylipins induce cell death in sea urchin embryos activating caspase-8 and caspase 3/7.
作者： Nadia Ruocco , Stefano Varrella , Giovanna Romano , Adrianna Ianora , Matt G. Bentley
DOI: 10.1016/J.AQUATOX.2016.04.012
关键词:
摘要: Diatoms are an important class of unicellular algae that produce bioactive secondary metabolites with cytotoxic activity collectively termed oxylipins, including polyunsaturated aldehydes (PUAs), hydroxyacids (HEPEs), oxo-acids and epoxyalcohols. Previous results showed at higher concentrations, the PUA decadienal induced apoptosis on copepods sea urchin embryos via caspase-3 activation; lower concentrations affected expression levels caspase-8 gene in Paracentrotus lividus. In present work, we studied effects other common oxylipins produced by diatoms: two PUAs (heptadienal octadienal) four (5-, 9- 11- 15-HEPE) P. lividus cell death caspase activities. Our (i) HEPEs embryos, detected microscopic observation through activation caspase-3/7 measured luminescent assays; (ii) low (isolated for first time here lividus) genes, Real Time qPCR. These findings have interesting implications from ecological point view, given importance diatom blooms nutrient-rich aquatic environments.
core.ac.uk
bournemouth.ac.uk参考文章(111)
Brian K. Hall, Masashi Hirata, Temporospatial patterns of apoptosis in chick embryos during the morphogenetic period of development. The International Journal of Developmental Biology. ,vol. 44, pp. 757- 768 ,(2000) , 10.1387/IJDB.11128569
Douglas R Green, Apoptotic Pathways: The Roads to Ruin Cell. ,vol. 94, pp. 695- 698 ,(1998) , 10.1016/S0092-8674(00)81728-6
H.C. Schröder, N. Janipour, W.E.G. Müller, G. Di Bella, R. Bonaventura, R. Russo, V. Matranga, DNA damage and developmental defects after exposure to UV and heavy metals in sea urchin cells and embryos compared to other invertebrates. Progress in molecular and subcellular biology. ,vol. 39, pp. 111- 137 ,(2005) , 10.1007/3-540-27683-1_6
Maria C Roccheri, Valeria Matranga, Rosa Bonaventura, Cinzia Tipa, Physiological and induced apoptosis in sea urchin larvae undergoing metamorphosis. The International Journal of Developmental Biology. ,vol. 46, pp. 801- 806 ,(2002) , 10.1387/IJDB.12382946
A. Miralto, G. Barone, G. Romano, S. A. Poulet, A. Ianora, G. L. Russo, I. Buttino, G. Mazzarella, M. Laabir, M. Cabrini, M. G. Giacobbe, The insidious effect of diatoms on copepod reproduction Nature. ,vol. 402, pp. 173- 176 ,(1999) , 10.1038/46023
Peter H. Krammer, CD95's deadly mission in the immune system Nature. ,vol. 407, pp. 789- 795 ,(2000) , 10.1038/35037728
Michael O. Hengartner, The biochemistry of apoptosis Nature. ,vol. 407, pp. 770- 776 ,(2000) , 10.1038/35037710
P. Provoost, V. Rousseau, K.E.R. Soetaert, J. Vanaverbeke, M. Steyaert, C. Lancelot, M.A. Franco, D. Van Oevelen, L. Moodley, D. Cox, M. Vincx, Benthic responses to sedimentation of phytoplankton on the Belgian Continental Shelf Current Status of Eutrophication in the Belgian Coastal Zone. pp. 73- 90 ,(2008)
The oxylipin chemistry of attraction and defense in brown algae and diatoms Natural Product Reports. ,vol. 19, pp. 108- 122 ,(2002) , 10.1039/A806888G
JT Bridgham, JA Wilder, H Hollocher, AL Johnson, All in the family: evolutionary and functional relationships among death receptors Cell Death & Differentiation. ,vol. 10, pp. 19- 25 ,(2003) , 10.1038/SJ.CDD.4401174