A triple-resonance pulse scheme for selectively correlating amide1HN and15N nuclei with the1Hα proton of the preceding residue
作者： Robert T. Clubb , Gerhard Wagner
DOI: 10.1007/BF01874816
关键词:
摘要: A 3D1H−15N−13C triple resonance experiment is presented that contains exclusively cross peaks between the1HN and15N nuclei of one residue with the Hα preceding residue. The pulse sequence, designed to minimize time coherence, transverse on short T2 values. consists coherence transfers via one-bond couplings from HN N, CO, Cα and back for detection; it called HN(COCA)HA. was tested uniformly15N- and13C-enriched T4 lysozyme.
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sci-hub.se 参考文章(19)
Lawrence P. McIntosh, A. Joshua Wand, David F. Lowry, Alfred G. Redfield, Frederick W. Dahlquist, Assignment of the backbone proton and nitrogen-15 NMR resonances of bacteriophage T4 lysozyme Biochemistry. ,vol. 29, pp. 6341- 6362 ,(1990) , 10.1021/BI00479A003
Kurt Wuthrich, NMR of proteins and nucleic acids ,(1986)
Gerhard Wagner, Kurt Wüthrich, Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra: Basic pancreatic trypsin inhibitor Journal of Molecular Biology. ,vol. 155, pp. 347- 366 ,(1982) , 10.1016/0022-2836(82)90009-2
Gaetano T. Montelione, Gerhard Wagner, Accurate measurements of homonuclear HN-H.alpha. coupling constants in polypeptides using heteronuclear 2D NMR experiments Journal of the American Chemical Society. ,vol. 111, pp. 5474- 5475 ,(1989) , 10.1021/JA00196A068
Dominique Marion, Mitsuhiko Ikura, Ad Bax, Improved solvent suppression in one-and two-dimensional NMR spectra by convolution of time-domain data Journal of Magnetic Resonance. ,vol. 84, pp. 425- 430 ,(1989) , 10.1016/0022-2364(89)90391-0
Gareth A. Morris, Ray Freeman, ENHANCEMENT OF NUCLEAR MAGNETIC RESONANCE SIGNALS BY POLARIZATION TRANSFER Journal of the American Chemical Society. ,vol. 101, pp. 760- 762 ,(1979) , 10.1021/JA00497A058
Edward T Olejniczak, Hugh L Eaton, Extrapolation of time-domain data with linear prediction increases resolution and sensitivity Journal of Magnetic Resonance. ,vol. 87, pp. 628- 632 ,(1990) , 10.1016/0022-2364(90)90321-Y
V.F. Bystrov, Spin—spin coupling and the conformational states of peptide systems Progress in Nuclear Magnetic Resonance Spectroscopy. ,vol. 10, pp. 41- 82 ,(1976) , 10.1016/0079-6565(76)80001-5
Lewis E Kay, Mitsuhiko Ikura, Ad Bax, The design and optimization of complex NMR experiments. Application to a triple-resonance pulse scheme correlating Hα, NH, and 15N chemical shifts in 15N13C-labeled proteins Journal of Magnetic Resonance. ,vol. 91, pp. 84- 92 ,(1991) , 10.1016/0022-2364(91)90410-U
Robert T. Clubb, V. Thanabal, Gerhard Wagner, A new 3D HN(CA)HA experiment for obtaining fingerprint H N -H α cross peaks in 15 N- and 13 C-labeled proteins Journal of Biomolecular NMR. ,vol. 2, pp. 203- 210 ,(1992) , 10.1007/BF01875531