3D triple-resonance NMR techniques for the sequential assignment of NH and 15N resonances in 15N- and 13C-labelled proteins.
作者： R�diger Weisemann , Heinz R�terjans , Wolfgang Bermel
DOI: 10.1007/BF00242479
关键词:
摘要: Two new 3D 1H-15N-13C triple-resonance experiments are presented which provide sequential cross peaks between the amide proton of one residue and nitrogen preceding succeeding residues or residues, respectively. These experiments, we term 3D-HN(CA)NNH 3D-H(NCA)NNH, utilize an optimized magnetization transfer via 2JNCα coupling to establish assignment backbone NH 15N resonances. In contrast NH-NH connectivities observable in homonuclear NOESY spectra, assignments from 3D-H(NCA)NNH experiment conformation independent a first-order approximation. Thus obtained these can be used as either confirmation conventional approach initial step analysis resonances according Ikura et al. (1990) [Biochemistry, 29, 4659–4667]. Both techniques were applied uniformly 15N- 13C-labelled ribonuclease T1.
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sci-hub.se 参考文章(22)
Dominique Marion, Lewis E. Kay, Steven W. Sparks, Dennis A. Torchia, Ad Bax, Three-dimensional heteronuclear NMR of nitrogen-15 labeled proteins Journal of the American Chemical Society. ,vol. 111, pp. 1515- 1517 ,(1989) , 10.1021/JA00186A066
Mitsuhiko Ikura, Ad Bax, G. Marius Clore, Angela M. Gronenborn, Detection of nuclear Overhauser effects between degenerate amide proton resonances by heteronuclear three-dimensional NMR spectroscopy Journal of the American Chemical Society. ,vol. 112, pp. 9020- 9022 ,(1990) , 10.1021/JA00180A080
Gerhard Wagner, Kurt Wüthrich, Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra: Basic pancreatic trypsin inhibitor Journal of Molecular Biology. ,vol. 155, pp. 347- 366 ,(1982) , 10.1016/0022-2836(82)90009-2
Gaetano T. Montelione, Gerhard Wagner, Accurate measurements of homonuclear HN-H.alpha. coupling constants in polypeptides using heteronuclear 2D NMR experiments Journal of the American Chemical Society. ,vol. 111, pp. 5474- 5475 ,(1989) , 10.1021/JA00196A068
Wayne Boucher, Ernest D. Laue, Sharon Campbell-Burk, Peter J. Domaille, Four-dimensional heteronuclear triple resonance NMR methods for the assignment of backbone nuclei in proteins Journal of the American Chemical Society. ,vol. 114, pp. 2262- 2264 ,(1992) , 10.1021/JA00032A053
Gareth A. Morris, Ray Freeman, ENHANCEMENT OF NUCLEAR MAGNETIC RESONANCE SIGNALS BY POLARIZATION TRANSFER Journal of the American Chemical Society. ,vol. 101, pp. 760- 762 ,(1979) , 10.1021/JA00497A058
Stephen W Fesik, Erik R.P Zuiderweg, Heteronuclear three-dimensional nmr spectroscopy. A strategy for the simplification of homonuclear two-dimensional NMR spectra Journal of Magnetic Resonance. ,vol. 78, pp. 588- 593 ,(1988) , 10.1016/0022-2364(88)90144-8
Robert T. Clubb, Gerhard Wagner, A triple-resonance pulse scheme for selectively correlating amide1HN and15N nuclei with the1Hα proton of the preceding residue Journal of Biomolecular NMR. ,vol. 2, pp. 389- 394 ,(1992) , 10.1007/BF01874816
Anil Kumar, G. Wagner, R.R. Ernst, K. Wüthrich, Studies of J-connectivities and selective 1H-1H Overhauser effects in H2O solutions of biological macromolecules by two-dimensional NMR experiments Biochemical and Biophysical Research Communications. ,vol. 96, pp. 1156- 1163 ,(1980) , 10.1016/0006-291X(80)90073-X
Lewis E Kay, Mitsuhiko Ikura, Ad Bax, The design and optimization of complex NMR experiments. Application to a triple-resonance pulse scheme correlating Hα, NH, and 15N chemical shifts in 15N13C-labeled proteins Journal of Magnetic Resonance. ,vol. 91, pp. 84- 92 ,(1991) , 10.1016/0022-2364(91)90410-U