Diversity of regulation of genetic transcription

摘要: Abstract The effect of antibiotics which prevent, either directly or indirectly, the formation polyribosomes (i.e. translocation ribosomes along messenger RNA), including fusidic acid, erythromycin, tetracycline, chloramphenicol and puromycin, on synthesis trp RNA bulk in Escherichia coli was studied. Quantitative estimates antibiotic-treated cells showed that, under conditions used, only about half species cell, RNA, subject to antibiotic effect. As dose each increased, over-all rate closely paralleled decreased protein synthesis, while continued be synthesized at control doses were sufficient block nearly all synthesis. Stimulation degradation occurred with puromycin but a small change half-life observed erythromycin. Chloramphenicol, acid tetracycline stabilized RNA. Evidence is presented showing that diminution detectable level attributable an arrest transcription rather than nascent molecules. In spite reduction cells, incorporation [3H]uridine into apparently not reduced some cases somewhat stimulated, due accelerated ribosomal hypothesis E. consists two types: one, coupled functional translational machinery; other independent such obligatory coupling.