Alteration in the expression of antioxidant and detoxification genes in Chironomus riparius exposed to zinc oxide nanoparticles.

摘要: Zinc oxide nanoparticles (ZnONPs) are widely used in several commercial products due to their unique physicochemical properties. However, release into the aquatic environments through various anthropogenic activities will lead toxic effect organisms. Although investigations have been reported on of ZnONPs organisms using traditional end points such as survival, growth, and reproduction, molecular level faster sensitive. In this study, expression different genes involved oxidative stress response, detoxification, cellular defense was studied an ecotoxicologically important bio-monitoring organism Chironomus riparius order understand subcellular effects ZnONPs. The fourth instar larvae were exposed 0, 0.2, 2, 10, 20 mg/L Zn ions (in form ZnSO4.7H2O) for 24 48 h period. CuZn superoxide dismutase, manganese catalase, phospholipid hydroperoxide glutathione peroxidase, thioredoxin reductase 1 delta-3, sigma-4 epsilon-1 classes S-transferases, cytochrome p4509AT2, heat shock protein 70 real-time polymerase chain reaction method. Gene results showed that related response more pronounced a result exposure compared ions. mRNA detoxification protection also modulated. Significantly higher levels stress-related shows is mechanism toxicity C. riparius.