Gene Expression Profiling in Ecotoxicology

摘要: Gene expression profiling is a powerful new end point for ecotoxicology and means bringing the genomics revolution to this field. We review usefulness of gene as an in describe methods applying approach non-model organisms. Since genomes contain thousands genes representing hundreds pathways, it possible identify toxicant-specific responses from wide array possibilities. Stressor-specific signatures profiles can be used diagnose which stressors are impacting populations Screening stress-induced requires special techniques organisms without sequenced genomes. These include differential display polymerase chain reaction (DD PCR), suppressive subtractive hybridization PCR (SSH representational difference analysis. model like yeast has identified that up-regulated response various stressors, including several well characterized (e.g., hsp78, metallothionein, superoxide dismutase). Using consensus primers animal sequences, amplify some these interest ecotoxicology. how grouped into cDNA arrays rapidly screening samples.