Functional peptide nanocarriers for delivery of novel anti-RelA RNA interference agents as a topical treatment of atopic dermatitis.

摘要: Abstract Small interfering RNAs (siRNAs) are a potential treatment of atopic dermatitis (AD) because they can specifically silence the gene expression AD-related factors. However, siRNA alone cannot exert sufficiently strong therapeutic effect due to low delivery efficiency target tissues and cells; simply increasing amount used is not possible possibility off-target effects. We previously reported novel class RNA interference (RNAi) agents called nkRNA ® PnkRNA , which have been shown be effective in several disease models, greater resistance nuclease degradation than canonical siRNAs, do induce any immunotoxicity. In present study, we describe non-invasive transdermal RNAi system for that uses functional cell-penetrating stearoyl-oligopeptide OK-102 as cytoplasm-responsive nanocarrier . The two were targeted against RelA, subclass NF-κB (nuclear factor kappa B), and, part complexes, strongly silenced RelA mRNA macrophage cells demonstrated significant mouse model AD. It was OK-102-complexed an efficient AD caused no adverse reactions.