The post-synaptic scaffolding protein tamalin regulates ligand-mediated trafficking of metabotropic glutamate receptors.

摘要: Group I metabotropic glutamate receptors (mGluRs) play important roles in various neuronal functions and have also been implicated multiple neuropsychiatric disorders like fragile X syndrome, autism, others. mGluR trafficking not only plays controlling the spatiotemporal localization of these cell but regulates activity receptors. Despite this obvious significance, cellular machineries that control group central nervous system studied detail. The post-synaptic scaffolding protein tamalin has shown to interact with mGluRs many other proteins involved neurons. Using a molecular replacement approach mouse hippocampal neurons, we show here critical role ligand-dependent internalization mGluR1 mGluR5, members family. Specifically, knockdown endogenous inhibited two Both N-terminal C-terminal regions played endocytosis. Furthermore, found by interacting S-SCAM, vesicular trafficking. Finally, demonstrate mGluR-mediated α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors, process believed be correlate for mGluR-dependent synaptic plasticity. Taken together, findings reveal mechanistic suggest its physiological implications brain.