Ultrasound-mediated disruption of cell membranes. I. Quantification of molecular uptake and cell viability

作者: Héctor R. Guzmán , Daniel X. Nguyen , Sohail Khan , Mark R. Prausnitz

DOI: 10.1121/1.1376131

关键词:

摘要: Ultrasound-mediated drug delivery is a nonchemical, nonviral, and noninvasive method for targeted transport of drugs genes into cells. Molecules can be delivered cells when ultrasound disrupts the cell membrane by mechanism believed to involve cavitation. This study examined molecular uptake viability in suspensions (DU145 prostate cancer aortic smooth musclecells) exposed varying peak negative acoustic pressures (0.6–3.0 MPa), exposure times (120–2000 ms), pulse lengths (0.02–60 ms) presence Optison (1.7% v/v) contrast agent. With increasing pressure time, marker compound, calcein, increased approached equilibrium with extra cellular solution, while decreased. Varying length produced no significant effect. All measurements collected over broad range conditions studied correlated energy exposure. suggests that may predictive ultrasound’s nonthermal bioeffects.

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