Prophage exotoxins enhance colonization fitness in epidemic scarlet fever-causing Streptococcus pyogenes.
作者： Stephan Brouwer , Timothy C. Barnett , Diane Ly , Katherine J. Kasper , David M. P. De Oliveira
DOI: 10.1038/S41467-020-18700-5
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摘要: The re-emergence of scarlet fever poses a new global public health threat. capacity North-East Asian serotype M12 (emm12) Streptococcus pyogenes (group A Streptococcus, GAS) to cause has been linked epidemiologically the presence novel prophages, including prophage ΦHKU.vir encoding secreted superantigens SSA and SpeC DNase Spd1. Here, we report molecular characterization ΦHKU.vir-encoded exotoxins. We demonstrate that streptolysin O (SLO)-induced glutathione efflux from host cellular stores is previously unappreciated GAS virulence mechanism promotes release activity, representing first description thiol-activated bacterial superantigen. Spd1 required for resistance neutrophil killing. Investigating single, double triple isogenic knockout mutants exotoxins, find act synergistically facilitate nasopharyngeal colonization in mouse model. These results offer insight into pathogenesis fever-causing mediated by
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